We are pleased to highlight a new collaborative review published in Current Opinion in Insect Science, co-authored with colleagues Fernando G. Noriega, Guy Bloch, Martin Moos, Petr Šimek, and Marek Jindra. The article, “Approaches to quantify and manipulate insect hormone signals,” provides an up-to-date overview of state-of-the-art methods used to study the key lipidic insect hormones—juvenile hormones (JHs) and ecdysteroids.
The review summarizes current mass-spectrometric approaches for highly sensitive detection of JHs and ecdysteroids, including validated LC-MS/MS workflows capable of quantifying hormone titers from minimal biological material. It also outlines contemporary chemical, genetic, and molecular tools that enable targeted enhancement or suppression of endocrine signaling, from synthetic agonists to CRISPR-based gene knockouts.
Our laboratory contributed expertise in analytical biochemistry and metabolomics, particularly in the development and application of LC-MS/MS methods for quantifying ecdysteroids at femtomolar concentrations.
