All Publications


  • 21-3 Gade G, Simek P, Marco H G (2021), Biochemically identified neuropeptides in a caddisfly (Trichoptera) and a pygmy mole cricket (Orthoptera: Caelifera: Tridactyloidea). Archives of Insect Biochemistry and Physiology 106 (2021), DOI: 10.1002/arch.21778

  • 21-2 Opekar S, Zahradnickova H, Vodrazka P, Rimnacova L, Simek P, Moos M (2021), A chiral GC-MS method for analysis of secondary amino acids after heptafluorobutyl chloroformate & methylamine derivatization. Amino Acids (2021), DOI: 10.1007/s00726-021-02949-1

  • 21-1 Rozsypal J, Moos M, Rudolf I, Kostal V (2021), Do energy reserves and cold hardiness limit winter survival of Culex pipiens? Comparative Biochemistry and Physiology, Part A 255 (2021), DOI: 10.1080/19476337.2020.1809525


  • 20-7 Potts LJ, Košťál V, Šimek P (2020), Energy balance and metabolic changes in an overwintering wolf spider, Scizocosa stridulans. Journal of Insect Physiology 126 (2020), DOI: 10.1016/j. jinsphys.2020.104112

  • 20-6 Heneberg P, Jegorov A, Šimek P (2020), Peroral administration of beauverolides allows their transfer to the peripheral blood and urine. CyTA - Journal of Food 18 (2020), 644-652, DOI: 10.1080/19476337.2020.1809525

  • 20-5 Šimčíková D, Tůma P, Jegorov A, Šimek P, Heneberg P (2020), Rapid methods for the separation of natural mixtures of beauverolides cholesterol acyltransferase inhibitors, isolated from the fungus Isaria fumosorosea. Journal of Separation Science 43 (2020), 962-969, DOI: 10.1002/jssc.201901084

  • 20-4 Gäde G, Šimek P, Marco H (2020), The adipokinetic peptides in Diptera: structure, function, and evolutionary trends. Frontiers in Endocrinology 11 (2020), DOI: 10.3389/fendo.2020.00153

  • 20-3 Li NG, Toxopeus J, Moos M, Sorensen J, Sinclair B (2020), A comparison of low temperature biology of Pieris rapae from Ontario, Canada, and Yakutia, Far Eastern Russia. Comparative Biochemistry and Physiology 242 (2020) DOI: 10.1016/j.cbpa.2020.110649

  • 20-2 Sam K, Koane B, Sam L, Mrázová A, Segar ST, Volf M, Moos M, Šimek P, Sisol M, Novotný V (2020), Insect herbivory and herbivores of Ficus species along a rain forest elevational gradient on Papua New Guinea. Biotropica 52 (2020), 263-276, DOI: 10.1111/btp.12741

  • 20-1 Škodová-Sveráková I, Prokopchuk G, Pena Diaz C, Záhonová K, Moos M, Horváth B, Šimek P, Lukeš J (2020), Unique Dynamics of Paramylon Storage in the Marine Euglenozoan Diplonema papillatum. Protist 171 (2020), DOI: 10.1016/j.protis.2020.125717


  • 19-5 Gäde G, Šimek P, Marco H (2019), Structural diversity of adipokinetic hormones in the hyperdiverse coleopteran Cucujiformia. Archives of Insect Biochemistry and Physiology 102 (2019), DOI: 10.1002/arch.21611

  • 19-4 Rozsypal J, Toxopeus J, Moos M, Berková P, Šimek P, Košťál V (2019), Fat body disintegration after freezing stress is a consequence rather than a cause of freezing injury in larvae of Drosophila melanogaster. Journal of Insect Physiology 115 (2019), 12-19, DOI: 10.1016/j.jinsphys.2019.03.008

  • 19-3 Štefanová I, Zápal J, Moos M, Kuzma M, Kubec R (2019), Isoalliin –derived thiolanes formed in homogenized onion. Journal of Agricultural and Food Chemistry 67 (2019), 9895-9906, DOI: 10.1021/acs.jafc.9b01384

  • 19-2 Šimek P, Hušek P, Zahradníčková H (2019), Heptafluorobutyl chloroformate-based sample preparation protocol for nonchiral and chiral amino acid analysis by gas chromatography-mass spectrometry. In: Alterman M. A. (ed.): Amino Acid Analysis: Methods and Protocols, Methods in Molecular Biology, Vol. 2030, Springer Science+Business Media, LLC, part of Springer Nature 2019, 455 pp., ISBN 978-1-4939-9638-4. 237-251. DOI: 10.1007/978-1-4939-9639-1_18

  • 19-1 Štětina T, Poupardin R, Moos M, Šimek P, Šmilauer P, Košťál V (2019), Larvae of Drosophila melanogaster exhibit transcriptional activation of immune response pathways and antimicrobial peptides during recovery from supercooling stress. Insect Biochemistry and Molecular Biology 105 (2019), 60-68, DOI: 10.1016/j.ibmb.2019.01.006


  • 18-8 Konečná M, Moos M, Zahradníčková H, Šimek P, Lepš J (2018), Tasty rewards for ants:differences in elaiosome and seed metabolite profiles are consistent across species and reflect taxonomic relatedness. Oecologia 188 (2018), 753–764, DOI: 10.1007/s00442-018-4254-8.

  • 18-7 Kubec R, Štefanová I, Moos M, Urajová P, Kuzma M, Zápal J (2018), Allithiolanes – nine groups of a newly discovered family of sulfur compounds responsible for the bitter off-taste of processed onion. Journal of agriculture and food chemistry 66 (2018), 8783–8794, DOI: 10.1021/acs.jafc.8b03118.

  • 18-6 Rozsypal J, Moos M, Goto SG (2018), Cold acclimation increases cold tolerance independently of diapause programing in the bean bug, Riptortus pedestris. Bulletin of Entomological Research 108 (2018), 83-92, DOI: 10.1017/S0007485317001006.

  • 18-5 Rozsypal J, Moos M, Šimek P, Košťál V (2018), Thermal analysis of ice and glass transitions in insects that do and do not survive freezing. Journal of Experimental Biology 221 (2018), DOI: 10.1242/jeb.170464.

  • 18-4 Hušek P, Švagera Z, Hanzlíková D, Karlínová I, Řimnáčová L, Zahradníčková H, Šimek P (2018), GC-MS metabolomic profiling of protic metabolites following heptafluorobutyl chloroformate mediated dispersive liquid microextraction sample preparation protocol; Georgios A. Theodoridis et al. (eds.), Metabolic Profiling: Methods and Protocols, Methods in Molecular Biology 2018, vol. 1738, pages 159-181, Chapter 11. © Springer Science+Business Media, LLC, part of Springer Nature 2018 DOI: org/10.1007/978-1-4939-7643-0_11.

  • 18-3 Štětina T, Hůla P, Moos M, (2018) Recovery from supercooling, freezing, and cryopreservation stress in larvae of the drosophilid fly, Chymomyza costata. Scientific Reports 8 (2018), DOI: 10.1038/s41598-018-22757-0.

  • 18-2 Volf M, Segar ST, Miller SE, Isua B, Sisol M, Aubona G, Šimek P, Moos M, Salminen J-P, Zima J, Jadranka Rota J, Weiblen GD, Wossa S, Novotny V (2018), Community structure of insect herbivores is driven by defensive traits with contrasting evolutionary dynamics: the role of trait escalation and divergence in Ficus. Ecology Letters 21 (2018), 83–92, DOI: 10.1111/ele.12875.

  • 18-1 Lehmann P, Pruisscher P, Košťál V, Moos M, Šimek P, Nylin S, Agren R, Väremo L, Wiklund C, Wheat CW, Gotthard K (2018), Metabolome dynamics of diapause in the butterfly Pieris napi: distinguishing maintenance, termination and post-diapause phases. Journal of Experimental Biology 221 (2018), DOI: 10.1242/jeb.169508.


  • 17-05 Řimnáčová L, Biology Centre CAS, New procedures in the metabolomic analysis of biofluids, PhD Thesis, Charles University, Faculty of Science, Prague 2017.

  • 17-04 Vrba P, Nedvěd O, Zahradníčková H, Konvička M (2017), More complex than expected: Cold hardiness and the concentration of cryoprotectants in overwintering larvae of five Erebia butterflies. European Journal of Entomology 114 (2017), 470-480, DOI: 10.14411/eje.2017.060.

  • 17-03 Vrba P, Nedvěd O, Zahradníčková H, Konvička M (2017), Temporal plasticity in cold hardiness and cryoprotectant contents in northern versus temperate Colias butterfies (Lepidoptera: Pieridae). Cryoletters 38 (2017), 330-338.

  • 17-02 Gäde G, Šimek P, Marco HG (2017), The African froghopper Ptyelus flavescens (suborder: Cicadomorpha) contains two novel and one known peptides of the adipokinetic hormone (AKH) family: structure, function and comparison with aphid AKH (suborder: Sternorrhyncha). Amino Acids 49 (2017), 1679-1690, DOI 10.1007/s00726-017-2461-y.

  • 17-01 Tomčala A, Kyselová V, Schneedorferová I, Opekarová I, Moos M, Urajová P, Kručinská J, Oborník M (2017), Separation and identification of lipids in the photosynthetic cousins of Apicomplexa Chromera velia and Vitrella brassicaformis. Journal of Separation Science 40 (2017), 3402-3413, DOI: 10.1002/jssc.201700171.


  • 16-11 Moos M., Šimek P., Petrus K., Hušek P, Robotic Sample Preparation Workstation MetaboAuto® – PGL HFBCF MXP-U. Applied Technology.

  • 16-10 Gäde G, Šimek P, Marco HG (2016), Novel members of the adipokinetic hormone family in beetles of the superfamily Scarabaeoidea. Amino Acids 48 (2016), 2785-2798, DOI: 10.1007/s00726-016-2134-0.

  • 16-09 Boardman L, Sørensen J G, Košťál V, Šimek P, Terblanche JS (2016), Chilling slows anaerobic metabolism to improve anoxia tolerance of insects. Metabolomics 12 (2016), 176-184, DOI: 10.1007/s11306-016-1119-1.

  • 16-08 Colinet H, Renault D, Javal M, Berková P, Šimek P, Košťál V (2016), Uncovering the benefits of fluctuating thermal regimes on cold tolerance of drosophila flies by combined metabolomic and lipidomic approach. BBA – Molecular and Cell Biology of Lipids 1861 (2016), 1736-1745.

  • 16-07 Boardman L, Sørensen JG, Košťál V, Šimek P and Terblanche JS (2016), Cold tolerance is unaffected by oxygen availability despite changes in anaerobic metabolism. Scientific Reports 6 (2016), 32856, DOI: 10.1038/srep32856.

  • 16-06 Košťál V, Korbelová J, Štětina T, Poupardin R, Colinet H, Zahradníčková H, Opekarová I, Moos M and Šimek P (2016), Physiological basis for low-temperature survival and storage of quiescent larvae of the fruit fly Drosophila melanogaster. Scientific Reports 6 (2016), DOI: 10.1038/srep32346.

  • 16-05 Košťál V, Korbelová J, Poupardin R, Moos M and Šimek P (2016), Physiological basis for low-temperature survival and storage of quiescent larvae of the fruit fly Drosophila melanogaster. Scientific Reports 6 (2016), 32346, DOI: 10.1038/srep32346.

  • 16-04 Mollaei M, Izadi H, Šimek P and Košťál V (2016), Overwintering biology and limits of cold tolerance in larvae of pistachio twig borer, Kermania pistaciella. Bulletin of Entomological Research 106 (2016), 538–545. DOI: 10.1017/S0007485316000237.

  • 16-03 Gäde G, Šimek P, Marco HG (2016), Identification and bioactivity evaluation of the first neuropeptide from the lesser‑known insect order Embioptera (webspinner). Amino Acids 48/7 (2016), 1677-1684, DOI: 10.1007/s00726-016-2229-9.

  • 16-02 Dean CAE, Nicolas M. Teets NM, Košťál V, Šimek P, Denlinger DL (2016), Enhanced stress responses and metabolic adjustments linked to diapause and onset of migration in the large milkweed bug Oncopeltus fasciatus. Physiological Entomology 41 (2016), 152–161. DOI: 10.1111/phen.12140.

  • 16-01 Hušek P, Švagera Z, Hanzlíková D, Řimnáčová L, Zahradníčková H, Opekarová I, Šimek P (2016), Profiling of urinary amino-carboxylic metabolites by in-situ heptafluorobutyl chloroformate mediated sample preparation and gas chromatography-mass spectrometry. Journal of Chromatography A 1443 (2016), 211-232. DOI: 10.1016/j.chroma.2016.03.019.


  • 15-07 Rozsypal J, Košťál V, Berková P, Zahradníčková H, Šimek P (2015), Reprint of: Seasonal changes in the composition of storage and membrane lipids in overwintering larvae of the codling moth, Cydia pomonella. Journal of Thermal Biology 54 (2015), 20–29. DOI: 10.1016/j.jtherbio.2015.10.007.

  • 15-06 Gäde G, Šimek P, Marco HG (2015),A novel adipokinetic peptide from the corpus cardiacum of the primitive caeliferan pygmy grasshopper Tetrix subulata (Caelifera, Tetrigidae). Peptides 68 (2015), 43-49. DOI: 10.1016/j.peptides.2015.01.008.

  • 15-05 Lehmann P, Kaunisto S, Košťál V, Zahradníčková H, Lindström L (2015), Comparative ecophysiology of cold-tolerance-related traits: assessing range expansion potential for an invasive insect at high latitude. Physiological and Biochemical Zoology 88 (2015), 254–265. DOI: 10.1086/680384.

  • 15-04 Gäde G, Šimek P, Marco HG (2015),Two novel tyrosine-containing peptides (Tyr4) of the adipokinetic hormone family in beetles of the families Coccinellidae and Silphidae. Amino Acids 47 (2015,) 2323-2333. DOI: 10.1007/s00726-015-2011-4.

  • 15-03 Klementová Š, Rabová-Toušová Z, Bláha L, Kahoun D, Šimek P, Keltnerová L, Zlámal M (2015), Photodegradation of atrazine on Ti02 –products toxicity assessment. Open Journal of Applied Sciences 5 (2015), 14-21. DOI: 10.4236/ojapps.2015.51002.

  • 15-02 Li Y, Zhang L, Chen H, Košťál V, Šimek P, Moos M, Denlinger DL (2015), Shifts in metabolomic profiles of the parasitoid Nasonia vitripennis associated with elevated cold tolerance induced by the parasitoid’s diapause, host diapause and host diet augmentation with proline. Insect Biochemistry and Molecular Biology63 (2015), 34-46. DOI: 10.1016/j.ibmb/2015.05.012.

  • 15-01 Tomek P, Hrouzek P, Kuzma M, Sýkora J, Fišer R, Černý J, Novák P, Bártová S, Šimek P, Hof M, Kavan D, Kopecký J (2015), Cytotoxic lipopeptide muscotoxin A, isolated from soil cyanobacterium Desmonostoc muscorum, permeabilizes phospholipid membranes by reducing their fluidity. Chemical Research in Toxicology 28 (2015), 216-224. DOI: 10.1021/tx500382b.


  • 14-08 Vrba P, Dolek M, Nedvěd O, Zahradníčková H, Cerrato C, Konvička M (2014), Overwintering of the boreal butterfly Colias palaeno in Central Europe. CryoLetters 35 (2014), 247-254.

  • 14-07 Paroulek M, Sláma K (2014), Production of the sesquiterpenoid, juvenile hormone-1 (JH-I), and of vitamin E in the accessory sexual (colleterial) glands of adult male moths, Hyalophora cecropia (Linnaeus, 1758), (Lepidoptera: Saturniidae). Life-The Excitement of Biology 2 (2014), 102-124. DOI: 10.9784/LEB2(2)Paroulek.01.

  • 14-06 Košťál V, Miklas B, Doležal P, Rozsypal J, Zahradníčková H (2014), Physiology of cold tolerance in the bark beetle, Pityogenes chalcographus and its overwintering in spruce stands. Journal of Insect Physiology 63 (2014), 62-70. DOI: 10.1016/j.jinsphys.2014.02.007.

  • 14-05 Kalová H, Janečková B, Verner M, Šimek P, Šintaj A, Šťastná H, Petr P (2014), Some biochemical, organoleptic and nutraceutical properties of wine with special emphasis on Moldovan wines: wine as medicamentum. Prevence úrazů, otrav a násilí 10 (2014), 38–52.

  • 14-04 Rozsypal J, Košťál V, Berková P, Zahradníčková H, Šimek P (2014), Seasonal changes in the composition of storage and membrane lipids in overwintering larvae of the codling moth, Cydia pomonella. Journal of Thermal Biology 45 (2014), 124-133. DOI: 10.1016/j.therbio.2014.08.011

  • 14-03 Marco HG, Šimek P, Gäde G (2014), Adipokinetic hormones of the two extant apterygotan insect orders, Archaeognatha and Zygentoma. Journal of Insect Physiology 60 (2014,) 17-24. DOI: 10.1016/j.jinsphys.2013.11.002.

  • 14-02 Řimnáčová L, Hušek P, Šimek P (2014), A new method for immediate derivatization of hydroxyl groups by fluoroalkyl chloroformates and its application for the determination of sterols and tocopherols in human serum and amniotic fluid by gas chromatography–mass spectrometry. Journal of Chromatography A, 1339 (2014), 154-167.

  • 14-01 Zahradníčková H, Tomčala A, Berková P, Schneedorferová I, Okrouhlík J, Šimek P, Hodková M (2014), Cost effective, robust, and reliable coupled separation techniques for the identification and quantification of phospholipids in complex biological matrices: application to insects. Journal of Separation Science 37 (2014), 2062-2068. DOI: 10.1002/jssc.201400113.


  • 13-05 Heather HG, Šimek P, Gäde G (2013), Novel adipokinetic hormones of the kissing bug Rhodnius prolixus, Triatoma infestans, Dipetalogaster maxima and Panstrongylus megist Peptides 41 (2013), 24-30.

  • 13-04 Košťál V, Urban T, Řimnáčová L, Berková P, Šimek P (2013), Seasonal changes in minor membrane phospholipid classes, sterols and tocopherols in overwintering insect, Pyrrhocoris apterus. Journal of Insect Physiology 59 (2013), 934-941.

  • 13-03 Gäde G, Šimek P, Clark KD, Marco HG (2013), Five functional adipokinetic peptides expressed in the corpus cardiacum of the moth genus Hippotion (Lepidoptera, Shingidae). Regulatory Peptides 184 (2012), 85-95.

  • 13-02 Košťál V, Rozsypal J, Pech P, Zahradníčková H, Šimek P (2013), Physiological and biochemical responses to cold and drought in the rock-dwelling pulmonate snail, Chondrina avenacea. Journal of Comparative Physiology B 183 (2012), 749-761.

  • 13-01 Rozsypal J, Košťál V, Zahradníčková H, Šimek P (2013), Overwintering strategy and mechanisms of cold tolerance in the codling moth (Cydia pomonella). PLoS One 8/4 (2013), e61745.


  • 12-12 Vesala L, Salminen TS, Košťál V, Zahradníčková H, Hoikkala A (2012), Myo-inositol as a main metabolite in overwintering flies: seasonal metabolomic profiles and cold stress tolerance in northern drosophilid fly. Journal of Experimental Biology 215 (2012), 2891-2897. DOI:10.1242/jeb.069948.

  • 12-11 Hrouzek P, Kuzma M, Černý J, Novák P, Fišer R, Šimek P, Štys D, Lukešová A, Kopecký J (2012), The cyanobacterial cyclic lipopeptides puwainaphycins F/G are inducing necrosis via cell membrane permeabilization and subsequent unusual act in relocalization. Chemical Research in Toxicology 25 (2012), 1203-1211. DOI: 10.1021/tx300044t.

  • 12-10 Hušek P, Švagera Z, Hanzlíková Z, Šimek P (2012), Survey of several methods deproteinizing human plasma before and within the chloroformate-mediated treatment of amino/carboxylic acids quantitated by gas chromatography. Journal of Pharmaceutical and Biomedical Analysis 67-68 (2012), 159-162. DOI: 10.1016/j.jpba.2012.04.027.

  • 12-09 Jedlička P, Steinbauerová V, Šimek P, Zahradníčková H (2012), Functional characterization of the adipokinetic hormone in the pea aphid, Acyrthosiphon pisum. Comparative Biochemistry and Physiology – Part A: Molecular & Integrative Physiology 162 (2012), 51-58. DOI: 10.1016/j.cbpa.2012.02.004.

  • 12-08 Weaver RJ, Marco HG, Šimek P, Audsley N, Clark KD, Gäde G (2012), Adipokinetic hormones (AKHs) of sphingid Lepidoptera, including the identification of a second Manduca sexta Peptides 34 (2012), 44-50. DOI: 10.1016/j.peptides.2012.01.009.

  • 12-07 Švagera Z, Hanzlíková Z, Šimek P, Hušek P (2012), Study of disulfide reduction and alkyl chloroformate derivatization of plasma sulfur amino acids using gas chromatography–mass spectrometry. Analytical and Bioanalytical Chemistry 402 (2012), 2953-2963. DOI: 10.1007/s00216-012-5727-y

  • 12-06 Fleischmannova J, Kucerova L, Sandova K, Steinbauerova V, Broz V, Simek P, Zurovec M (2012), Differential response of Drosophila cell lines to extracellular adenosine. Insect Biochemistry & Molecular Biology 42 (2012), 321-331. DOI:10.1016/j.ibmb.2012.01.002.

  • 12-05 Košťál V, Šimek P, Zahradníčková H, Cimlová J, Štětina T (2012), Conversion of the chill susceptible fruit fly larva (Drosophila melanogaster) to a freeze tolerant organism. Proceedings of the National Academy of Sciences USA 109 (2012), 3270-3274. DOI: 10.1073/pnas.1119986109.

  • 12-04 Vranova V, Zahradnickova H, Janous D, Skene KR, Avtar S. Matharu AS, Rejsek K, Formanek P (2012), The significance of D-amino acids in soil, fate and utilization by microbes and plants: review and identification of knowledge gaps. Plant and Soil 354 (2012), 21–39, DOI 10.1007/s11104-011-1059-5.

  • 12-03 Hušek P (2012), Letter to the editor regarding GC–MS with ethyl chloroformate derivatization for comprehensive analysis of metabolites in serum and its application to human uremia. Analytical and Bioanalytical Chemistry 403 (2012), 2758-2786, DOI 10.1007/s00216-011-5615-x.

  • 12-02 Cimlová J, Kružberská P, Švagera Z, Hušek P, Šimek P (2012), In situ derivatization-liquid liquid extraction as a sample preparation strategy for the determination of urinary biomarker prolyl-4-hydroxyproline by liquid chromatography–tandem mass spectrometry. Journal of Mass Spectrometry 47 (2012), 294-302. DOI:10.1002/jms.2952.

  • 12-01 Šimek P, Hušek P, Zahradníčková H (2012), Heptafluorobutyl chloroformate – based sample preparation protocol for nonchiral and chiral analysis of amino acids by gas chromatography. Methods in Molecular Biology 828 (2012), 137-152. Alterman N.A., Hutzinger P. eds., Amino Acid Handbook, Chapter 13, Springer Media & Bussiness Media, New York 2012. DOI: 10.1007/978-1-61779-445-2_13.


  • 11-08 Gäde G, Šimek P, Marco HG (2011), An invertebrate [hydroxyproline]-modified neuropeptide: further evidence for a close evolutionary relationship between insect adipokinetic hormone and mammalian gonadotropin hormone family. Biochemical and Biophysical Research Communications 414 (2011), 592-597.

  • 11-07 Košťál V, Korbelová J, Zahradníčková H, Cimlová J, Tomčala A, Šimek P (2011), Long-term cold acclimation extends survival time at 0°C and modifies the metabolomic profiles of the larvae of the fruit fly Drosophila melanogaster. PlosOne 6 (2011), e25025.DOI : 10.1371/journal.pone.0025025

  • 11-06 Košťál V, Zahradníčková H, Šimek P (2011), Hyperprolinemic larvae of drosophilid fly, Chymomyza costata, survive cryopreservation in liquid nitrogen. Proceedings of the National Academy of Sciences USA 108 (2011), 13041-13046. DOI: 10.1073/pnas.1107060108.

  • 11-05 Košťál V, Doležal P, Rozsypal J, Moravcová M, Zahradníčková H, Šimek P (2011), Physiological and biochemical analysis of overwintering and cold tolerance in two Central, European populations of the spruce bark beetle Ips typographus. Journal of Insect Physiology 57 (2011), 1136-1146. DOI: o10.1016/j.jinsphys.2011.03.011.

  • 11-04 Kubec R, Krejcova P, Simek P, Vaclavík L, Hajslova J, Schraml J (2011), Precursors and formation of pyrithione and other pyridyl-containing sulfur compounds in drumstick onion, Allium stipitatum. Journal of Agricultural and Food Chemistry 59 (2011), 5763-5770. DOI: 10.1021/jf200704n.

  • 11-03 Marco HG, Šimek P, Gäde G (2011), The first decapeptide adipokinetic hormone (AKH) in Heteroptera; a novel AKH from a South African saucer bug, Laccocoris spurcus (Naucoridae, Laccocorinae). Peptides 32 (2011), 454-460.

  • 11-02 Kučerová P, Kubec R, Šimek P, Václavík L, Schraml J (2011), Allium discoloration: The precursor and formation of the red pigment in giant onion (Allium giganteum Regel) and some other subgenus Melanocrommyum species. Journal of Agricultural and Food Chemistry 59 (2011), 1821-1828.

  • 11-01 Gäde G, Šimek P, Fescemyer HW (2011), Adipokinetic hormones provide interference for the phylogeny of Odonata. Journal of Insect Physiology 57 (2011), 174-178.


  • 10-06 Zuberova M, Fenckova M, Šimek P, Janeckova L, Dolezal T (2010), Increased extracellular adenosine in adenosine deaminase deficient flies activates a release of energy stores leading to wasting and death. Disease Models & Mechanisms 3 (2010), 773-784.

  • 10-05 Bártů I, Tomčala A, Socha R, Šimek P, Kodrík D (2010), Analysis of lipids mobilized by adipokinetic hormones in the firebug Pyrrhocoris apterus (L.). European Journal of Entomology 107 (2010), 509-520.

  • 10-04 Kodrík D, Marco HG, Šimek P, Socha R, Štys P, Gäde G (2010), The adipokinetic hormones of Heteroptera: a comparative study. Physiological Entomology 35 (2010), 117-127.

  • 10-03 Gäde G, Šimek P (2010), A novel member of the adipokinetic peptide family in a ‘‘living fossil’’, the ice crawler Galloisiana yuasai, is the first identified neuropeptide from the order Grylloblattodea. Peptides 31 (2010), 372–376.

  • 10-02 Zralá J, Kodrík D, Zahradníčková H, Zemek R, Socha R (2010), A novel function of red pigment-concentrating hormone in crustaceans: Porcellio scaber (Isopoda) as a model species. General and Comparative Endocrinology 166 (2010), 330-336.

  • 10-01 Tomčala A, Bártů I, Šimek P, Kodrík D (2010), Locust adipokinetic hormones mobilize diacylglycerols selectively. Comparative Biochemistry and Physiology Part B 156 (2010), 26-37.


  • 09-02 Zahradníčková H, Hušek P, Šimek P (2009), GC separation of amino acid enantiomers via derivatization with heptafluorobutyl chloroformate and Chirasil-L-Val column. Journal of Separation Science 32 (2009), 3919-3924. IF= 2.655.

  • 09-01 Gäde G, Šimek P, Marco HG (2009),The first identified neuropeptide in the insect order Megaloptera: A novel member of the adipokinetic hormone family in the alderfly Sialis lutaria. Peptides 30 (2009), 477– 482. IF = 2.565.


  • 08-10 Hulak M, Paroulek M, Šimek P, Kocour M, Gela D, Rodina M, Linhart O (2008),Water polluted by 17α-methyltestosterone provides successful male sex inversion of common carp (Cyprinus carpio) from gynogenetic offspring. Journal of Applied Ichtyology 24 (2008), 707-710. IF = 0.638.

  • 08-09 Hušek P, Švagera Z, Všianský F, Franeková J, Šimek P (2008), Prolyl-hydroxyproline dipeptide in non-hydrolyzed morning urine and its value in postmenopausal osteoporosis. Clinical Chemistry and Laboratory. Medicine 46 (2008), 1391-1397. IF = 2.654.

  • 08-08 Zahradníčková H, Hartvich P, Šimek P, Hušek P (2008), Gas chromatographic analysis of amino acid enantiomers in Carbetocin peptide hydrolysates after fast derivatization with pentafluoropropyl chloroformate. Amino Acids 35 (2008), 445-450. IF = 4.132.

  • 08-07 Šimek P, Hušek P, Zahradníčková H (2008), Gas chromatographic-mass spectrometric analysis of biomarkers related to folate and cobalamin status in human serum after dimercaptopropanesulfonate reduction and heptafluorobutyl chloroformate derivatization. Analytical Chemistry 80 (2008), 5776-5782. IF = 5.712.

  • 08-06 Gäde G, Marco HG, Šimek P, Audsley N, Clark KD, Weaver RJ (2008), Predicted versus expressed adipokinetic hormones, and other small peptides from the corpus cardiacum–corpus allatum: A case study with beetles and moths. Peptides 29 (2008), 1124-1139. IF = 2.565.

  • 08-05 Overgaard J, Tomčala A, Soronsen JG, Holmstrup M, Krogha PH, Šimek P, Košťál V (2008), Effects of acclimation temperature on thermal tolerance and membrane phospholipid composition in the fruit fly Drosophila melanogaster. Journal of Insect Physiology 54 (2008), 619-629. IF = 2.155.

  • 08-04 Hušek P, Šimek P, Hartvich P, Zahradníčková H (2008), Fluoroalkyl chloroformates in treating amino acids for gas chromatographic analysis. Journal of Chromatography A 1186 (2008), 391-400. IF = 3.758.

  • 08-03 Zahradníčková H, Jegorov A, Trnka T, Zelenka K (2007), Thiosugars – Derivatization agents for chiral resolution of homoleucines. Journal of Separation Science 31 (2008), 133-136. IF = 2.655.

  • 08-02 Mráz J, Dušková S, Šimek P, Marečková J, Nohová H, Stránský V (2008), Biological monitoring of exposure to ethylene oxide in the sterilization units. Chemické listy 101 (2008), 234-236. IF = 0.593.

  • 08-01 Streinz L, Hartvich P, Ondráček J, Šimek P, Fejfarová K, Dušek M (2008), (R)-2-{2-[(S)-(20Benzoyloxy-1,10-binaphthyl-2-yl)oxycarbonylamino]-3-phenylpropanamidomethyl} pyridinium picrate acetone solvate. Acta Crystallographica E 64 (2008), 326-327, suppl. 1-13. IF = 0.367.


  • 07-05 Popkov A, Nadvornik M, Kružberska P, Lyčka A, Lehel S, Gillings N (2007), Towards stereoselective radiosynthesis of alpha-[C-11]methylsubstituted aromatic alpha-amino acids a challenge of creation of quaternary asymmetric centre in a very short time. Journal of Labelled Compounds & Radiopharmaceuticals 50 (2007), 370-374.

  • 07-04 Gäde G, Šimek P, Marco HG (2007), Water scorpions (Heteroptera, Nepidae) and giant water bugs (Heteroptera, Belostomatidae): Sources of new members of the adipokinetic hormone/red pigment-concentrating hormone family. Peptides, 28 (2007), 1359-1367.

  • 07-03 Zahradníčková H, Hušek P, Šimek P, Hartvich P, Maršálek B, Holoubek I (2007), Determination of D- and L-amino acids produced by cyanobacteria using gas chromatography on Chirasil-Val after derivatization with pentafluoropropyl chloroformate. Analytical and Bioanalytical Chemistry 388 (2007), 1815-1822. IF = 3.328.

  • 07-02 Košťál V, Zahradníčková H, Šimek P, Zelený J (2007), Multiple component system of sugars and polyols in the overwintering spruce bark beetle, Ips typographus. Journal of Insect Physiology 53 (2007), 580–586.

  • 07-01 Gäde G, Šimek P, Marco HG (2007), A novel adipokinetic peptide in a water boatman (Heteroptera, Corixidae) and its bioanalogue in a saucer bug (Heteroptera, Naucoridae). Peptides 28 (2007), 594-601.


  • 06-08 Otová B, Václavíková R, Danielová V, Holubová J, Ehrlichová M, Horský S, Souček P, Šimek P, Gut I (2006), Effects of paclitaxel, docetaxel and their combinations on subcutaneous lymphomas in inbred Sprague-Dawley/Cub rats. European Journal of Pharmaceutical Sciences 29 (2006), 442-450.

  • 06-07 Tomčala A, Tollarová M, Overgaard J, Šimek P, Košťál V (2006), Seasonal acquisition of chill tolerance and restructuring of membrane glycerophospholipids in an overwintering insect: triggering by low temperature, desiccation and diapause progression. Journal of Experimental Biology 209 (2006), 4102-4114.

  • 06-06 Gut I, Ojima I, Vaclavíkova R, Simek P, Horsky S, Linhart I, Soucek P, Kondrova E, Kuznetsova LV, Chen J (2006), Metabolism of new-generation taxanes in human pig, minipig, and rat liver microsomes. Xenobiotica 36 (2006), 772-792.

  • 06-05 Galis I, Šimek P, Narisawa T, Sasaki M, Horiguchi T, Fukuda H, Matsuoka K, A novel R2R3 MYB transcription factor NtMYBJS1 is a methyl jasmonate-dependent regulator of phenylpropanoid-conjugate biosynthesis in tobacco. The Plant Journal 46 (2006), 573-592.

  • 06-04 Gäde G, Šimek P, Clark K, Auerswald L (2006), Unique translational modification of an invertebrate neuropeptide: a phosphorylated member of the adipokinetic hormone peptide family. Biochemical Journal 393 (2006), 705-713.

  • 06-03 Hušek P, Šimek P (2006), Alkyl chloroformates in sample derivatization strategies for GC Analysis. Current Pharmaceutical Analysis 2 (2006), 21-41.

  • 06-02 Zahradníčková H, Bouman E (2006), Optimization of the solid-phase microextraction method in the determination of Ixodes ricinus (L.) volatiles. Journal of Separation Sciences 29 (2006), 236-241.

  • 06-01 Mráz J, Cimlová J, Stránský V, Nohová H, Kičová R, Šimek P, N-Methylcarbamoyl-lysine adduct in globin: a new metabolic product and potential biomarker of N,N-dimethylformamide in humans. Toxicology Letters 162 (2006), 211-218.


  • 05-04 Hušek P (2005), Quantitation of amino acids as chloroformates – A return to gas chromatography, in: Quantitation of amino acids and amines by chromatography, ed. I. Molnár-Perl, Elsevier, Amsterdam, p. 2-38, 2005.

  • 05-03 Doleželová E, Žurovec M, Doležal T, Šimek P, Bryant PJ (2005), The emerging role of adenosine deaminases in insects. Insect Biochemistry and Molecular Biology 35 (2005), 381-389.

  • 05-02 Gäde G, Marco HG, Šimek P, Marais E (2005) The newly discovered insect order mantophasmatodea contains a novel member of the adipokinetic hormone family of peptides. Biochemical and Biophysical Research Communnications 330 (2005), 598-603.

  • 05-01 Zahradníčková H, Hartvich P, Holoubek I (2005), Historie a význam chirálních analýz aminokyselin v biologických matricích a životním prostředí. Chemické Listy 99 (2005), 703-710.


  • 04-6 Košťál V, Tamura M, Tollarová M, Chvalová D, Zahradníčková H (2004), Enzymatic capacity for accumulation of polyol cryoprotectants changes during diapause development in the adult red firebug Pyrrhocoris apterus. Physiological Entomology 29 (2004), 344-355.

  • 04-5 Slaninová J, Bennettová B, Nazarov ES, Šimek P, Holík J, Vlasáková V, Hlaváček V, Černý J, Tykva R (2004), Activity and mechanism of action of insect oostatic peptides in flesh fly. Bioorganic Chemistry 32 (2004), 263-273.

  • 04-4 Socha R, Kodrík D, Šimek P, Patočková M (2004), The kind of AKH-mobilized energy substrates in insects can be predicted without a knowledge of the hormone structure. European Journal of Entomology 101 (2004), 29-35.

  • 04-3 Mráz J, Šimek P, Chvalová D, Nohová H, Šmigolová P (2004), Studies on the methyl isocyanate adducts with globin. Chemico-Biological Interactions 148 (2004), 1-10.

  • 04-2 Václavíková R, Souček P, Svobodová L, Anzenbacher P, Šimek P, Guengerich FP, Gut I (2004), Different in vitro metabolism of paclitaxel and docetaxel in humans, rats, pigs and minipigs. Drug Metabolism and Disposition 32 (2004), 666-674 IF= 3.277.

  • 04-1 Gális I, Kakiuchi Y, Šimek P, Wabiko H (2004), Agrobacterium tumefaciens AK-6b gene modulates phenolic compound metabolism in tobacco. Phytochemistry 65 (2004), 169-179, IF=1.686.


  • 03-7 Košťál V, Berková P, Šimek P (2003), Remodelling of membrane phospholipids during transition to diapause and cold-acclimation in the larvae of Chymomyza costata (Drosophilidae). Comparative Biochemistry and Physiology B–Biochemistry & Molecular Biology 135 (2003), 407-419.

  • 03-6 Hušek P, Matucha P, Vránková A, Šimek P (2003), Simple plasma work-up for a fast chromatographic analysis of homocysteine, cysteine, methionine and aromatic amino acids. Journal of Chromatography B 789 (2003), 311-322. IF=1.913.

  • 03-5 Bouman EAP, Dusbábek F, Šimek P, Zahradníčková H (2003), Methyl 3-chloro-4-methoxybenzoate, a new candidate semiochemical inhibiting copulation behaviour of Ixodes ricinus (L.) males. Physiological Entomology 28 (2003), 276-282. IF=1.280.

  • 03-4 Hronková M, Zahradníčková H, Šimek P (2003), The role of abscisic acid in acclimation of plants cultivated in vitro to ex vitro Biologia Plantarum 46 (2003), 535-541.

  • 03-3 Hušek P, Šimek P, Matucha P (2003), Smoth esterification of di- and tricarboxylic acids with methyl and ethyl chloroformates in gas chromatographic profiling of urinary acidic metabolites. Chromatographia 58 (2003), 623-630. IF=1.230.

  • 03-2 Gäde G, Auerswald L, Šimek P, Marco HG, Kodrík D (2003), Red pigment concentrating hormone is not limited to crustaceans. Biochemical and Biophysical Research Communications 309 (2003), 967-973. IF=2.935.

  • 03-1 Václavíková R, Horský D, Šimek P, Gut I, (2003)), Paclitaxel metabolims in rat and human liver microsomes is inhibited by phenolic antioxicants. Naunyn-Schmiedeberg’s Archives in Pharmacology 368 (2003), 200-209. IF=2.566.


  • 02-7 Šlachta M, Vambera J, Zahradníčková H, Košťál V (2002), Entering diapause is a prerequisite for successful cold-acclimation in adult Graphosoma lineatum (Heteroptera: Pentatomidae). Journal of Insect Physiology 48 (2002), 1031-1039.

  • 02-6 Hodková M, Berková P, Zahradníčková H (2002), Photoperiodic regulation of the phospholipid molecular species composition in thoracic muscles and fat body of Pyrrohocoris apterus (Heteroptera) via an endocrine gland, corpus allatum. Journal of Insect Physiology 48 (2002), 1009-1019. IF=1.49.

  • 02-5 Gális I, Šimek P, Van Onckelen HA, Kakiuchi Y, Wabiko H (2002), Resistance of transgenic seedlings expressing the Agrobacterium tumefaciens C58-6b gene, to growth-inhibitory levels of cytokinin is associated with elevated IAA levels and activation of phenylpropanoid metabolism. Plant and Cell Physiology 43 (2002), 939-950, IF=2.430.

  • 02-4 Hušek P, Šimek P, Tvrzická E (2002) Simple and rapid procedure for determination of individual fatty acids in serum. Analytica Chimica Acta 465 (2002), 433-439, IF=2.073.

  • 02-3 Mráz J, Dušková Š, Gálová E, Nohová H, Šimek P, Linhart I (2002), Improved gas chromatographic–mass spectrometric determination of N-methylcarbamoyl adduct at the N-terminal valine of globin, a metabolic product of the solvent N, N-dimethylformamide. Journal of Chromatography B 778 (2002), 357-365, IF=1.911.

  • 02-2 Šlachta M, Berková P, Vambera J, Košťál V (2002), Physiology of cold acclimation in the non-diapausing adults of Pyrrhocoris apterus (Heteroptera). European Journal of Entomology 99 (2002), 181-187, IF=0.802.

  • 02-1 Kodrík D, Šimek P, Lepša L, Socha R (2002), Identification of the cockroach neuropeptide Pea-CAH-II as a second adipokinetic hormone in the firebug Pyrrhocoris apterus. Peptides, 23 (2002), 585-587. IF=1.867.


  • 01-3 Hušek P, Šimek P (2001), Advances in amino acid analysis. LC GC North America 19 (2001), 986-999. IF=3.665.

  • 01-2 Košťál V, Šlachta M, Šimek P (2001), Cryoprotective role of polyols independent of the increase in supercooling capacity in diapausing adults of Pyrrhocoris apterus (Heteroptera: Insecta). Comparative Biochemistry and Physiology B 130 (2001), 365-374, IF=0.831.

  • 01-1 Košťál V, Havelka J, Šimek P (2001), Low-temperature storage and cold hardiness in two populations of the predatory midge Aphidoletes aphidimyza, differing in diapause intensity. Physiological Entomology 26 (2001), 320-328, IF=0.931.


  • 00-5 Tolman V, Šimek P (2000), Chemistry of 4-fluoroglutamic acid. Part 4. Resolution of the racemic erythro and threo forms through the diastereoisomeric salts. Journal of Fluorine Chemistry 101 (2000), 1-14. IF=0.851.

  • 00-4 Kodrík D, Socha R, Šimek P, Goldsworthy GJ (2000), A new member of the AKH/RPCH family that simulates locomotory activity in the firebug Pyrrhocoris apterus (Heteroptera). Insect Biochemistry and Molecular Biology 30 (2000), 489-498. IF=1.920.

  • 00-3 Koštál V, Šimek P (2000), Overwintering strategy in Pyrrhocoris apterus (Heteroptera): The relations between life-cycle, chill tolerance and physiological adjustments. Journal of Insect Physiology 46 (2000), 1321-1329. IF=1.468.>/p>

  • 00-2 Jegorov A, Cvak L, Hušek A, Šimek P, Heydová A, Ondráček J, Pakhomová S, Hušák M, Kratochvíl B, Sedmera P, Havlíček V (2000), Synthesis and crystal structure determination of cyclosporin H. Collection of Czechoslovak Chemical Communications 65 (2000), 1317-1326. IF=0.960.

  • 00-1 Kozák L, Francová H, Hrabincová E, Procházková D, Juttnerová V, Bzduch V, Šimek P (2000), Smith-Lemli-Opitz syndrome: Molecular-genetic analysis of ten families. Journal of Inherited Metabolic Diseases 23 (2000), 409-412. IF=1.414.


  • 99-2 Hodková M, Šimek P, Zahradníčková H, Nováková O (1999), Seasonal changes of fatty acid composition of phospholipids in thoracic muscles of a heteropteran, Pyrrhocoris apterus. Insect Biochemistry and Molecular Biology 29 (1999), 367-376.

  • 99-1 Gális I, Šimek P, Macas J, Zahradníčková H, Vlasák J, Wabiko H, Van Dongen W, Van Onckelen H, Ondřej M (1999), The Agrobacterium tumefaciens C58-6b gene confers resistance to N6-benzyl-adenine without modifying cytokinin metabolism in tobacco seedlings. Planta 209 (1999), 453-461.


  • 98-3 Dusbábek F, Zahradníčková H, Šimek P (1998), Chemical stability of assembly pheromone of argasid ticks (Ixodoidea: Argasidae). Folia Parasitologica 45 (1998), 62-66.

  • 98-2 Košťál V, Šula J, Šimek P (1998), Physiology of drought tolerance and cold hardiness of the mediterranean tiger moth Cymbalophora pudica during summer diapause. Journal of Insect Physiology 44 (1998), 165-173.

  • 98-1 Košťál V, Šimek P (1998), Changes in fatty acid composition of phospholipids and triacylglycerols after cold acclimation of an aestivating insect prepupa. Journal of Comparative Physiology B – Biochemical Systemic and Environmental Physiology168 (1998), 453-460.


  • 97-2 Jegorov A, Šimek P, Heydová A, Cvak L, Minář V (1997), Free and bonded homoisoleucine in sclerotia of parasitic fungus Claviceps purpurea. Amino Acids 12 (1997), 9-19.

  • 97-1 Dusbábek F, Rupeš V, Šimek P, Zahradníčková H (1997), Enhancement of permethrin efficacy in acaricide-attractant mixtures for control of the fowl tick Argas persicus (Acari: Argasidae). Experimental and Applied Acarology 21 (1997), 293-305.


  • 96-2 Košťál V, Šimek P (1996), Biochemistry and physiology of aestivo-hibernation in the adult apple blossom weevil, Anthonomus pomorum (Coleoptera: Curculionadae). Journal of Insect Physiology 42 (1996), 727-733.

  • 96-1 Košťál V, Nedvěd O, Šimek P (1996), Accumulation of high concentrations of myo-inositol in the overwintering ladybird beetle Ceratomegilla undecimnotata. CryoLetters 17 (1996), 267-272.


  • 95-2 Jegorov A, Maťha V, Sedmera P, Havlíček V, Stuchlík J, Seidel P, Šimek P (1995), New natural cyclosporines from Tolypocladium terricola. Phytochemistry 38 (1995), 403-407.

  • 95-1 Košťál V, Šimek P (1995), Dynamics of cold hardiness, supercooling and cryoprotectants in diapausing and non-diapausing pupae of the cabbage root fly, Delia radicum Journal if Insect Physiology 41 (1995), 627-634.


  • 94-6 Šimek P, Heydová A, Jegorov A (1994), High resolution capillary gas chromatography and GC/MS of protein and nonprotein amino acids, amino alcohols and hydroxycarboxylic acids as their tert-butyldimethylsilyl derivatives. HRC – Journal of High Resolution Chromatography 17 (1994), 147-152.

  • 94-5 Šimek P, Jegorov A, Dusbábek F (1994), Determination of purine, pyrimidine bases and nucleosides in excreta of Arthropods by conventional and microbore HPLC and gas chromatography with mass spectrometric detection. Journal of Chromatography A 679 (1994), 195-200.

  • 94-4 Kadlec Z, Šimek P, Heydová A, Jegorov A, Maťha V, Landa Z, Eyal J (1994), Chemotaxonomic discrimination among the fungal genera Tolypocladium, Beauveria and Paecilomyces. Biochemical Systematics and Ecology 22 (1994), 803-806.

  • 94-3 Jegorov A, Sedmera P, Maťha V, Šimek P, Zahradníčková H, Landa Z, Eyal J (1994), Beauverolides L and La from Beauveria tenella and Paecilomyces fumosoroseum. Phytochemistry 37 (1994), 1301-1303.

  • 94-2 Zahradníčková H, Šimek P, Hořicová P, Tříska J (1994), Determination of atrazine and simazine in drinking and surface waters by solid phase extraction and high performance thin layer chromatography. Journal of Chromatography A 688 (1994), 383-389.

  • 94-1 Šimek P, Heydová A, Jegorov A (1994), Amino Acids, Amino Alcohols, Beauverolides, Hydroxycarboxylic acids. Recorder (Lovendegem, Belgium) 90, (1994) 461-463, 479.