The project is focused on the research and development of proprietary technology for fully automated analysis of small molecules in biological samples. The aim of the project is to develop a comprehensive tool for metabolomic analysis, which begins by inserting sample vials with a biological fluid or tissue extract in a robotic device, followed by fully automatic sample work-up and analysis. Sample preparation will be fully integrated with the standardized column separation of substances coupled with mass spectrometric detection and subsequent fully automatic processing / data extraction and assessment of the sample composition. The whole process thus will take place automatically. For successful implementation, a robotic device integrates with an autosampler capability will be designed and constructed after research and integration of all sample operations ranging from mixing, centrifugation, transfer of media, metabolites labeling with new original agents, those labeled with stable isotopes up to the evaporation, dilution and safety measures. Data measured on standard instruments will be developed and exploited by using the developed platform Metabolite Mapper, which will allow automatic data export into standard formats and processing using commercially available statistical tools. The successful achievement of project objectives will require a comprehensive approach and close cooperation between experts of basic research with experts in mechanical and automated systems, software engineering, information technology and skilled communication with experts in systems of quality control and with potential customers. The proposed project will create a new fully automated tool for efficient metabolite mapping and will contribute to the new knowledge of biological materials on a qualitatively new level. This all will contribute to the overall objectives of the ALFA Programme.
Download application note with methodology and list of compounds here: